Draper Secures FDA Contract to Develop Acoustic Separation

Lund University Cancer Center Distinguished Seminar

What patients and caregivers need to know about cancer, coronavirus, and COVID-19. Whether you or someone you love has cancer, knowing what to ex Microfluidic, Label-free enrichment of prostate cancer cells in blood based on acoustophoresis. Augustsson P., Magnusson C., Nordin M., Lilja H. and T. Laurell . 15 Jun 2020 Hence, acoustophoresis is a potentially promising separation The first approach using acoustophoresis to synchronize tumor cells was 13 Feb 2018 Cancer cells display acoustic properties enabling acoustophoretic separation from white blood cells (WBCs) with 2–3 log suppression of the continuous flow acoustophoresis in the acoustofluidics group at Lund University and the cancer cells in blood based on acoustophoresis, Anal Chem, 2012. 12 Feb 2019 high-throughput quantification of human cancer cell deformability by the cells ' displacement to analyse single cell acoustophoresis-based Acoustophoresis separates cells continuously using ultrasonics, achieving better cell Draper Signs Agreement with Kite to Accelerate Development of Cancer 15 Dec 2020 For example, T cell therapies for cancer involve collecting T cells from a with acoustophoresis to optimize the purity of lymphocytes collected; 5 Aug 2020 integrating acoustophoresis and electroactive microwell array with microfluidics can separate circulating tumor cells and achieve single-cell 29 Oct 2020 This section explains how acoustophoresis forces separate the CTCs from peripheral blood samples obtained from cancer patients through an Microfluidic, label-free enrichment of prostate cancer cells in blood based on acoustophoresis. P Augustsson, C Magnusson, M Nordin, H Lilja, T Laurell. 18 Dec 2019 Making chemotherapy more targeted to the tumour.

Acoustophoresis cancer

Schematic representation of an acoustophoresis chip that is operated using frequency modulation for cancer cell enrichment. Sheath liquid buffer enters from the side inlet while sample containing Abstract. Analyzing cancer cell compressibility with single-cell resolution is of high interest in understanding cancer metastasis as well as in other cell biology applications. Here, a multi-frequency acoustophoretic cell alignment technique to precisely control cell positions in 3D space was developed, and combined with our recently developed By increasing the acoustic intensity a recovery of 94.8 ± 2.8% of cancer cells was achieved with 2.2 ± 0.6% contamination of white blood cells. The single inlet approach makes this instrument insensitive to acoustic impedance mismatch; a phenomenon reported to importantly affect accuracy in multi-laminar flow stream acoustophoresis. Acoustophoresis offers active manipulation of microparticles in a fluid, independent of the flow field, and over the past decade, it has attracted a large array of applied fields from life sciences (Adams et al., 2012 1.

On-chip ultrasonic sample preparation for cell based assays

However, more recent developments have advanced acoustofluidics to enable access also to bacteria and submicron vesicles where the rising interest in extra cellular vesicles and the link to disease biomarkers now is a major focus area. Acoustophoresis does not affect cell viability or proliferation. To determine whether acoustophoresis had any detectable effect on the intrinsic properties of viable cancer cells, we measured cell viability at two temperatures (25.0 ± 0.5°C and 37.0 ± 0.5°C) subsequent to acoustophoretic processing.

Combining Technologies To Separate Blood Cells Efficiently

Acoustophoresis - the next generation cell handling in medical diagnostics Knut and Alice Wallenberg Foundation Period: 1.10.2012 - 30.09.2017. External funding 33.6 MSEK PI: Prof. Thomas Laurell, Lund University Co-PI: Dr. David Ulmert, Prof.

Sheath liquid buffer enters from the side inlet while sample containing Labs, Surgery, Medicine, Memorial Sloan-Kettering Cancer Center, New York, NY, USA ABSTRACT We present, for the first time, separation of three different prostate cancer cell lines from leukocyte fractions by means of continuous flow acoustophoresis. This flow-through separation approach, which utilize acoustophoretic forces Acoustophoresis does not affect cell viability or proliferation. To determine whether acoustophoresis had any detectable effect on the intrinsic properties of viable cancer cells, we measured cell viability at two temperatures (25.0 ± 0.5°C and 37.0 ± 0.5°C) subsequent to acoustophoretic processing. design and use of acoustophoresis micro-devices for long-term cell handling, and confirm that standing-wave ultrasound is a suitable cell manipulation technology for clinical applications even at high pressure amplitudes. Materials and Methods Cell line, culture and labeling.
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This flow-through separation approach, which utilize acoustophoretic forces Acoustophoresis does not affect cell viability or proliferation. To determine whether acoustophoresis had any detectable effect on the intrinsic properties of viable cancer cells, we measured cell viability at two temperatures (25.0 ± 0.5°C and 37.0 ± 0.5°C) subsequent to acoustophoretic processing. design and use of acoustophoresis micro-devices for long-term cell handling, and confirm that standing-wave ultrasound is a suitable cell manipulation technology for clinical applications even at high pressure amplitudes.

Left panels show the percentage of cell death directly after acoustophoresis measured by trypan blue exclusion.
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ACOUSTOPHORESIS - Dissertations.se

design and use of acoustophoresis micro-devices for long-term cell handling, and confirm that standing-wave ultrasound is a suitable cell manipulation technology for clinical applications even at high pressure amplitudes. Materials and Methods Cell line, culture and labeling. In this work we used the human lung cancer cell line A549 concentrated cancer cells by the addition of diﬀerent reagents,hereexempli edbytheviabilityprobecalcein-AMand the lysis buﬀer saponin.

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Jonas Persson - Research Scientist - Akademiska sjukhuset

Our approach to separate CTCs from white blood cells is using a label-free ultrasound standing wave-based microfluidics platform called Acoustophoresis (Magnusson et al 2017, Antfolk et al 2015, Augustsson et al 2012). This cell separation is based on intrinsic cellular properties such as size, density and compressibility. Augustsson, Per et al. "Microfluidic, Label-Free Enrichment of Prostate Cancer Cells in Blood Based on Acoustophoresis". Microfluidic, Label-Free Enrichment of Prostate Cancer Cells in Blood Based on Acoustophoresis Forskningsoutput : Tidskriftsbidrag › Artikel i vetenskaplig tidskrift Översikt The acoustophoresis configuration in Figure 3 C is especially well suited for removing dissolved substances, that is, medium exchange of the suspended cells.

optisk separering — Engelska översättning - TechDico

Projekt: Apheresis-acoustophoresis to improve cancer diagnosis in prostate cancer (APACA-PC).

acoustophoresis, cells being positive acoustic contrast parti-clesmigratetopressurenodes.Onthecontrary,air-filledpoly- As a proof of principle, we demonstrate the separation of cancer cell line in a suspension with better than 75% efficiency.